Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Assay Development, Bioassay, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 42705

View all CXCR1/IL-8RA Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
Met1-Leu350
Accession # AAA59159

Specificity

Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCR1/IL-8RA Antibody

Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody.

Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330). The ND₅₀ is typically 0.4-2.0 µg/mL.

Detection of CXCR1/IL-8RA by Flow Cytometry

IL‐8/CXCR1 contributes to stemness in vitro. (A) Effect on sphere formation of single agent or combination treatment with human recombinant IL‐8, anti‐CXCR1 antibody and/or repertaxin for Caki‐1 (Kruskal–Wallis test, n = 5). (B) Effect on sphere formation of single agent or combination treatment with human recombinant IL‐8, anti‐CXCR1 antibody and repertaxin for 769P cells (Kruskal–Wallis test, n = 5). (C) Flow cytometry analysis of the SP and the CXCR1+ cell compartment upon repertaxin treatment in the spheres derived from Caki‐1. (D) Histograms showing decreased SP and CXCR1+ cells upon repertaxin treatment. The yellow area indicates number of events with verapamil treatment. The red area shows CSC and CXCR1+ populations in the untreated sample. The blue area displays the remaining number of events after repertaxin treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30883740), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CXCR1/IL-8RA by Western Blot

IL-8 as a partial inducer of MMP9 in ESCC cells. (A): ELISA quantification of IL-8 in culture supernatants of ESCC cells after direct co-culture with macrophages. Directly co-cultured ESCC cells secreted more IL-8 than monocultured counterparts. (B): Western blot revealed the expression of known IL-8 receptors, CXCR1 (45 kDa, monomer; 80–90 kDa, dimer and their glycosylated forms) and CXCR2 (41 kDa) in TE-9, TE-10, and TE-11 cell lines. (C,D): Treatment with rhIL-8 (100 ng/mL, for 24 h) upregulated MMP9 mRNA expression in all three ESCC cell lines (C) but only triggered MMP9 secretion from TE-10 (D). Data are expressed as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001. NS, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37296952), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CXCR1/IL-8RA by Western Blot

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Applications for Human CXCR1/IL-8RA Antibody

Application

Recommended Usage

CyTOF-reported

Cheng, Y. et al. (2016) J. Immunol. 196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human whole blood granulocytes

Neutralization

Measured by its ability to neutralize CXCL8/IL-8-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR1. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL-8.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB330 in the following applications:

Block/Neutralize (1 Review)
Immunohistochemistry (3 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR1/IL-8RA

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Gene Symbol

CXCR1

UniProt

AAA59159

Additional CXCR1/IL-8RA Products

Product Documents for Human CXCR1/IL-8RA Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CXCR1/IL-8RA Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry