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Recombinant Human IL-8/CXCL8 Protein

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Human IL-8/CXCL8 Protein Bioactivity
(2)
Recombinant Human IL-8/CXCL8 Protein SDS-PAGE
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With Carrier
208-IL-050
208-IL-010

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Carrier Free
208-IL-010/CF
208-IL-050/CF
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Key Product Details

Source: E. coli
Accession #: P10145.1
Applications: Bioactivity

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Citations for Recombinant Human IL-8/CXCL8 Protein (122)

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Customer Reviews for Recombinant Human IL-8/CXCL8 Protein (6)

4.8 out of 5
6 customer ratings
5 stars
83%
4 stars
17%
3 stars
0%
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  • Recombinant Human IL-8/CXCL8 Protein
    Name: Anonymous
    Application: Cell migration/motility
    Reason for Rating: The human primary cells were treated with IL-8 to test its effect on cell migration. The IL-8 protein was used as an agonist and it worked nicelly.
    Verified Customer | Posted 03/15/2021
    The primary cells were starved overnight and treated with IL-8 (40ng/ml) and tested its effects on cell migration.
    Recombinant Human IL-8/CXCL8 Protein Cell migration/motility 208-IL
  • Recombinant Human IL-8/CXCL8 Protein
    Name: Anonymous
    Application: Assay validation
    Verified Customer | Posted 02/09/2021
  • Recombinant Human IL-8/CXCL8 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/03/2018
    rhIL-8 induce beta -arrestin release in CXCR1/U2OS reporter cell line
    Recombinant Human IL-8/CXCL8 Protein In vitro bioactivity in cell culture 208-IL
  • Recombinant Human IL-8/CXCL8 Protein
    Name: Zoia Levashova
    Application: Immunoassay Standard
    Verified Customer | Posted 11/26/2018
    The recombinant human IL-8 protein was used as the calibration reagent in MSD assay with biotinylated MAB208 (capture) and Sulfo-Tag labeled AF-208-NA (detection) antibodies.
    Recombinant Human IL-8/CXCL8 Protein Immunoassay Standard 208-IL
  • Recombinant Human IL-8/CXCL8 Protein
    Name: Anonymous
    Application: activation of neutrophils from blood in vitro.
    Reason for Rating: It worked well for the activation of neutrophils.
    Verified Customer | Posted 11/10/2017
  • Recombinant Human IL-8/CXCL8 Protein
    Name: Michelle Chen
    Application: Neutrophils were activated with 208-IL and assessed for morphological changes and migratory activity on 2D cell culture plate.
    Verified Customer | Posted 04/07/2017
    Recombinant Human IL-8/CXCL8 Protein Other 208-IL

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FAQs for Recombinant Human IL-8/CXCL8 Protein

  • Are mouse or rat CXCL8/IL-8 products offered?

    Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

  • What is the difference between 208-IL (rhCXCL8/IL-8) and  618-IL (rhEndothelial Cell-Derived IL-8/CXCL8)?

    Several N-terminal processed forms of CXCL8/IL-8 are produced by proteolytic cleavage after secretion by cells such as peripheral blood monocytes, leukcocytes and endothelial cells. Catalog # 208-IL consists only of the sequence Ser28 to Ser99 (also known as IL-8, 6-77), which is the most prominent form of IL-8. According to literature, IL-8(6-77) has a 5-10-fold higher activity in neutrophil activation. Catalog # 618-IL consists of Ser28 to Ser99 (IL-8, 6-77) and Ala23 to Ser99 (IL-8).  In our chemoattraction assay, 208-IL has a 2-fold higher activity than 618-IL.Several N-terminal processed forms of CXCL8/IL-8 are produced by proteolytic cleavage after secretion by cells such as peripheral blood monocytes, leukcocytes and endothelial cells. Catalog # 208-IL consists only of the sequence Ser28 to Ser99 (also known as IL-8, 6-77), which is the most prominent form of IL-8. According to literature, IL-8(6-77) has a 5-10-fold higher activity in neutrophil activation. Catalog # 618-IL consists of Ser28 to Ser99 (IL-8, 6-77) and Ala23 to Ser99 (IL-8).  In our chemoattraction assay, 208-IL has a 2-fold higher activity than 618-IL.

  • Can Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL) be used with mouse cells?

    Bioactivity of Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL) has only been evaluated using human cells transfected with human CXCR2 (the human receptor for IL-8), and we have not evaluated the activity of this protein using mouse cells. There is no IL-8/CXCL8 gene counterpart in rodent species. We are aware of a few publications citing use of 208-IL in mouse assay systems, so we would recommend reviewing the publications listed on the Citations tab of the product-specific web page to find reported use of our products in similar experimental layouts.

Product Documents for Recombinant Human IL-8/CXCL8 Protein

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