Detects human CXCL7/NAP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 10% cross-reactivity with recombinant human (rh) GRO alpha, rhIL-8, rhCINC-2 alpha, and rhGRO beta is observed, and 5% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta, rhGCP-2, rmKC, rhMIG, and rhGRO beta. is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CXCL7/NAP-2 Antibody

Detection of Human CXCL7/NAP‑2 by Simple Western^TM.

Simple Western lane view shows lysates of human blood platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP-2 at approximately 7 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL7/NAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human CXCL7 / NAP-2 ELISA Standard Curve

Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP) was serially diluted and captured by Mouse Anti-Human CXCL7/NAP-2 Monoclonal Antibody (Catalog # MAB393) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL7/NAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CXCL7/NAP-2 Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: Human blood platelets

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/NAP-2

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP‑III) and beta-thrombogulin ( beta‑TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

References

Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.

Alternate Names

CTAP III, NAP-2, NAP2, PPBP

Entrez Gene IDs

5473 (Human)

Gene Symbol

PPBP

UniProt

P02775

Additional CXCL7/NAP-2 Products

Product Documents for Human CXCL7/NAP-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CXCL7/NAP-2 Antibody

For research use only

Citations
Reviews

Human CXCL7/NAP-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF393

Key Product Details

Species Reactivity

Validated:

Cited:

Applications