Human CXCL7/NAP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB393
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Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Simple Western
Cited:
Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture), Functional Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 59418
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL7/NAP-2
Ala59-Asp128
Accession # P02775
Ala59-Asp128
Accession # P02775
Specificity
Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCL7/NAP-2 Antibody
Myeloperoxidase Release Induced by CXCL7/NAP-2 and Neutralization by Human CXCL7/NAP-2 Antibody.
Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP) induces myeloperox-idase release in the cytochalasin-B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/ NAP-2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15-20 µg/mL in the presence of cytochalasin-B.
Detection of Human CXCL7/NAP-2 by Simple WesternTM.
Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human CXCL7 / NAP-2 ELISA Standard Curve
Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP) was serially diluted and captured by Mouse Anti-Human CXCL7/NAP-2 Monoclonal Antibody (Catalog # MAB393) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL7/NAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CXCL7/NAP-2 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human platelets
Sample: Human platelets
Western Blot
1 µg/mL
Sample: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)
Sample: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)
Neutralization
Measured by its ability to neutralize CXCL7/NAP-2-induced myeloperoxidase release in cytochalasin-B treated human neutrophils. Schröder, J. M. et al. (1987) J. Immunol. 139:3474. The Neutralization Dose (ND50) is typically 15-20 µg/mL in the presence of 1 µg/mL Recombinant Human CXCL7/NAP-2 and cytochalasin-B.
Human CXCL7/NAP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB393 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/NAP-2
Additional CXCL7/NAP-2 Products
Product Documents for Human CXCL7/NAP-2 Antibody
Product Specific Notices for Human CXCL7/NAP-2 Antibody
For research use only
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