Key Product Details
Product Summary for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody
Accession # O43927
Scientific Data Images for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody
Detection of CXCL13/BLC/BCA‑1 in Immature Dendritic cells by Flow Cytometry.Immature Dendritic cells (CD14+ selected PBMCs are treated with 20 ng/mL Recombinant Human IL-4 Protein (204-IL) and 50 ng/mL Recombinant Human GM-CSF Protein (215-GM) for 6 days) were stained with Mouse Anti-Human CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB1777A) and either (A) Mouse Anti-Human CXCL13/BLC/BCA‑1 PE‑conjugated Monoclonal Antibody (Catalog # IC8012P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Applications for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: Immature dendritic cells (CD14+ selected PBMCs are treated with 20 ng/mL Recombinant Human IL-4 Protein (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF Protein (Catalog # 215-GM) for 6 days)
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
Product Specific Notices for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody
For research use only