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Human Corin Antibody

Catalog # MAB2209 | R&D Systems, Inc. a Bio-Techne Brand
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MAB2209-100
MAB2209-500
MAB2209-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 231443

Product Summary for Human Corin Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Corin
Ser67-Asn1042
Accession # Q9Y5Q5

Specificity

Detects human Corin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Corin Antibody

Corin antibody in HK2 Human Cell Line by Immunocytochemistry (ICC).

Corin in HK2 Human Cell Line.

Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human Corin Antibody in ELISA Standard Curve.

Human Corin ELISA Standard Curve.

Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Corin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Corin DuoSet ELISA Kit (Catalog # DY2209) for convenient development of a sandwich ELISA or the Human Corin Quantikine ELISA Kit (Catalog # DCRN00) for a complete optimized ELISA.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HK2 human kidney proximal tubule cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB2209 in the following applications:

Published Applications

Read 1 publication using MAB2209 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Corin

Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP.

Alternate Names

ATC2, Pro-ANP-Converting Enzyme

Entrez Gene IDs

10699 (Human)

Gene Symbol

CORIN

UniProt

Product Documents for Human Corin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Corin Antibody

For research use only

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Reconstitution Calculator

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