Recombinant Human CNDP2/CPGL Protein, CF

Name: Recombinant Human CNDP2/CPGL Protein, CF
Brand: R&D Systems
SKU: 3560-ZN

R&D Systems, part of Bio-Techne | Catalog # 3560-ZN

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Product Datasheet / COA / SDS

Discontinued Product

3560-ZN has been discontinued. View all Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL products.

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q96KP4
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human CNDP2/CPGL Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human CNDP2/CPGL Protein, CF

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL protein Met1-Asp475, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser299 (C-terminal fragment) & Ala2 predicted (full length & N-terminal fragment)
Predicted Molecular Mass	54 kDa (full-length), 33 kDa (N-terminal fragment) and 21 kDa (C-terminal fragment)
SDS-PAGE	54 kDa (full-length), 33 kDa (N-terminal fragment) and 21 kDa (C-terminal fragment)
Activity	Measure by its ability to cleave carnosine ( beta -Ala-His) in a two-step assay. The specific activity is >70 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3560-ZN

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL

The human CNDP2 gene encodes cytosol non-specific dipeptidase, a member of the M20 family of metalloproteases (1, 2). Also known as glutamate carboxypeptidase-like protein 1 (CPGL), tissue carnosinase or peptidase A, CNDP2 is ubiquitously expressed throughout human tissues. Unlike CNDP1, CNDP2 has broad substrate specificity for dipeptides with free amino and carboxyl groups. The purified recombinant human CNDP2 migrates as three bands in SDS-PAGE under reducing conditions that correspond to the full length protein (54 kDa), the N-terminal fragment (33 kDa) and the C-terminal fragment (22 kDa). The initiator Met1 may be removed and Ala2 may be acetylated based on Accession # Q96KP4.

Item	Value
References	Teufel, M. et al. (2003) J. Biol. Chem. 278:6521. Woessner, J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp.1020, Academic Press, San Diego.
Entrez Gene IDs	55748 (Human)
Alternate Names	CN2, CN2cytosolic non-specific dipeptidase, CNDP dipeptidase 2, CNDP dipeptidase 2 (metallopeptidase M20 family), CNDP2, CPGL, CPGLcytosolic nonspecific dipeptidase, FLJ10830, HsT2298, PEPA, PEPAEC 3.4.13.18, Peptidase AGlutamate carboxypeptidase-like protein 1

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Recombinant Human CNDP2/CPGL Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3560-ZN

Key Product Details

Product Specifications for Recombinant Human CNDP2/CPGL Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL

Customer Reviews for Recombinant Human CNDP2/CPGL Protein, CF

Product Documents for Recombinant Human CNDP2/CPGL Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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