HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CLEC14A Monoclonal Antibody (Catalog # MAB7436, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

CLEC14A in HUVEC Human Cells.

CLEC14A was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human CLEC14A Monoclonal Antibody (Catalog # MAB7436) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CLEC14A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC14A

CLEC14A (C-type Lectin domain family 14 member A), also known as EGFR5, is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. It is a type I transmembrane protein, apparently expressed in brain. Mature human CLEC14A is 469 amino acids (aa) in length. It contains a 376 aa extracellular region (aa 22-397) and a 72 aa cytoplasmic domain. The extracellular region shows one C-type lectin like domain (aa 32-175) and an EGF-like region (aa 245-287). Over aa 22-397, human CLEC14A shares 66% and 81% aa identity with mouse and canine CLEC14A, respectively.

Long Name

C-type Lectin Domain Family 14, Member A

Alternate Names

CEG1, EGF R5

Entrez Gene IDs

161198 (Human); 66864 (Mouse); 314148 (Rat)

Gene Symbol

CLEC14A

UniProt

Q86T13

Additional CLEC14A Products

Product Documents for Human CLEC14A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CLEC14A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CLEC14A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7436

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CLEC14A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CLEC14A Antibody

Detection of CLEC14A in HUVEC Human Cells by Flow Cytometry.