Human CD47 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4670P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 472603

View all CD47 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722

Specificity

Detects human CD47. Stains human CD47 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD47 PE-conjugated Antibody

Detection of CD47 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD47 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 PE-conjugated Monoclonal Antibody (Catalog # FAB4670P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD47 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Reviewed Applications

Read 1 review rated 5 using FAB4670P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD47

CD47, also known as integrin-associated protein, is a ubiquitous 50 kDa multipass transmembrane protein with a single IgV-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternate splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Gene Symbol

CD47

UniProt

Q08722

Additional CD47 Products

Product Documents for Human CD47 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD47 PE-conjugated Antibody

For research use only

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