Human CD39/ENTPD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11713
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3304A
Product Specifications
Immunogen
Synthetic peptide
Accession # P49961
Accession # P49961
Specificity
Detects recombinant human CD39/ENTPD1 protein in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CD39/ENTPD1 Antibody
Detection of CD39 in Human Brain Cortex via seqIF™ staining on COMET™
CD39 was detected in immersion fixed paraffin-embedded sections of human Brain Cortex using Rabbit Anti-Human CD39, Monoclonal Antibody (Catalog# MAB11713) at 20ug/mL at 37°Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of endothelial cells. Protocol available in COMET™ Panel Builder.
Detection of Human CD39/ENTPD1 by Western Blot.
Western Blot shows lysates of human lung, human placenta and HDLM-2 human Hodgkin’s lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human CD39/ENTPD1 Monoclonal Antibody (Catalog # MAB11713) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD39/ENTPD1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of CD39/ENTPD1 in Human Breast Tumor.
CD39/ENTPD1 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Rabbit Anti-Human CD39/ENTPD1 Monoclonal Antibody (Catalog # MAB11713) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Applications for Human CD39/ENTPD1 Antibody
Application
Recommended Usage
Immunohistochemistry
1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast tumor, human brain cortex and human lung tumor
Sample: Immersion fixed paraffin-embedded sections of human breast tumor, human brain cortex and human lung tumor
Multiplex Immunofluorescence
20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Simple Western
10 µg/mL
Sample: HDLM-2 human Hodgkin's lymphoma cell line
Sample: HDLM-2 human Hodgkin's lymphoma cell line
Western Blot
2 µg/mL
Sample: Human lung, human placenta and HDLM-2 human Hodgkin's lymphoma cell line
Sample: Human lung, human placenta and HDLM-2 human Hodgkin's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39/ENTPD1
Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. rhNTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta- and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thrombo regulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).
References
- Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
- Rowe, M. et al. (1982) Int. J. Cancer 29:373.
- Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
- Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
- Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
- Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Alternate Names
ADPase, ATP-diphosphatase, CD39, ENTPD1, NTPD1
Gene Symbol
ENTPD1
UniProt
Additional CD39/ENTPD1 Products
Product Documents for Human CD39/ENTPD1 Antibody
Product Specific Notices for Human CD39/ENTPD1 Antibody
For research use only
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