Human CD36/SR-B3 Antibody

Name: Human CD36/SR-B3 Antibody
Brand: R&D Systems
SKU: AF1955

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1955

Citations (7)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36 Gly30-Asn439 Accession # P16671
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human CD36 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse CD36 is observed.

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Applications for Human CD36/SR-B3 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10-50 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD36/SR-B3 Antibody

	Western Blot Detection of Human CD36/SR‑B3 by Western Blot. Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human CD36/SR‑B3 by Simple Western^TM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Simple Western Detection of Human CD36/SR‑B3 by Simple Western^TM. Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta -amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

Item	Value
References	Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. Husemann, J. et al. (2002) Glia 40:195. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Long Name	Scavenger Receptor Class B, Member 3
Entrez Gene IDs	948 (Human); 12491 (Mouse)
Alternate Names	BDPLT10, CD36, CD36 antigen, CD36 antigen (collagen type I receptor, thrombospondin receptor), CD36 molecule (thrombospondin receptor), CD_antigen: CD36, CHDS7, Collagen R, FAT, Fatty acid translocase, GP3B, GP4, GPIIIb, GPIV, Glycoprotein IIIb, PAS IV, PAS-4, PAS-4 protein, PASIV, Platelet collagen receptor, SCARB3, SR-B3, SRB3, Thrombospondin R, Thrombospondin receptor, cluster determinant 36, platelet glycoprotein 4, scavenger receptor class B, member 3

Citations for Human CD36/SR-B3 Antibody (7)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD36/SR-B3 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

J Chean et al. (2021), Human CEACAM1-LF regulates lipid storage in HepG2 cells via fatty acid transporter CD36 The Journal of Biological Chemistry, 0(0):101311.
PMID: 34666041
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Suhail et al. (2019), DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells Cell Rep, 29(11):3522-3538.e7.
PMID: 31825833
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Ladanyi et al. (2018), Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis Oncogene, 0(0).
PMID: 29398710
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Samovski D et al. (2015), Regulation of AMPK activation by CD36 links fatty acid uptake to beta-oxidation. Diabetes, 64(2):353-9.
PMID: 25157091
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Allred CC et al. (2011), A novel ELISA for measuring CD36 protein in human adipose tissue. J. Lipid Res., 52(2):408-15.
PMID: 21115967
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Xu G et al. (2009), Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis. Circ. Res., 105(5):500-10.
PMID: 19644050
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Mahadevan VS et al. (2006), Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity. Cardiovasc. Res., 72(1):60-8.
PMID: 16860294

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Showing 1 - 7 of 7 citations Showing All

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