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Human CD36/SR‑B3 Antibody

Catalog # MAB19554 | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.
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MAB19554-100
MAB19554-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1283D

Product Summary for Human CD36/SR‑B3 Antibody

Immunogen

Human CD36/SR-B3 synthetic peptide
Accession # P16671

Specificity

Detects human CD36/SR-B3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CD36/SR‑B3 Antibody

Detection of Human CD36/SR-B3 antibody by Western Blot.

Detection of Human CD36/SR‑B3 by Western Blot.

Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19554) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD36/SR-B3 antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

CD36/SR‑B3 in U937 Human Cell Line.

CD36/SR-B3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19554) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
CD36/SR-B3 antibody in Human Heart by Immunohistochemistry (IHC-P).

CD36/SR‑B3 in Human Heart.

CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19554) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD36/SR‑B3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

1 µg/mL
Sample: Human placenta tissue and human platelets
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells,
beta‑amyloid fibrils (fA beta), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References

  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  2. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. 
  3. Husemann, J. et al. (2002) Glia 40:195.
  4. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
  5. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.

Long Name

Scavenger Receptor Class B, Member 3

Alternate Names

CD36, Collagen R, FAT, GPIIIb, GPIV, SCARB3, SR-B3, SRB3, Thrombospondin R

Entrez Gene IDs

948 (Human); 12491 (Mouse)

Gene Symbol

CD36

UniProt

Product Documents for Human CD36/SR‑B3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD36/SR‑B3 Antibody

For research use only

Reconstitution Calculator

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