Human CD25/IL-2R alpha Antibody

Name: Human CD25/IL-2R alpha Antibody
Brand: R&D Systems
SKU: AF-223-NA
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-223-NA

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(3)

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Key Product Details

Species Reactivity:	Human
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Knockout Validated, Western Blot
Label:	Unconjugated
Specificity:	Detects human CD25/IL‑2 R alpha in direct ELISAs and Western blots.

View Full Technical Details for Human CD25/IL-2R alpha Antibody

Applications for Human CD25/IL-2R alpha Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	Human whole blood lymphocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Item	Value
Neutralization	Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
Knockout Validated	CD25/IL-2R alpha is specifically detected in HDLM‑2 human Hodgkin's lymphoma cell line parental cell line but is not detectable in CD25/IL-2R alpha knockout HDLM‑2 human Hodgkin's lymphoma cell line cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD25/IL-2R alpha Antibody

	Western Blot Detection of Human CD25/IL-2R alpha by Western Blot. Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line and PC-3 human prostate cancer cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). GAPDH (AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Knockout Validated Western Blot Shows Human CD25/IL-2R alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and human CD25/IL-2 R alpha knockout HDLM‑2 human Hodgkin’s lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated) in the parental HDLM‑2 human Hodgkin’s lymphoma cell line cell line, but is not detectable in knockout HDLM‑2 human Hodgkin’s lymphoma cell line cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND₅₀ is typically 0.2-1 µg/mL.

Formulation, Preparation and Storage

Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

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Background: CD25/IL-2R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Item	Value
Long Name	Interleukin 2 Receptor alpha
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names	CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL-2Ra, IL2-RA, IL2R, IL2R alpha, IL2RA, TAC antigen, TCGFR, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55

Citations for Human CD25/IL-2R alpha Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD25/IL-2R alpha Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Zheng CF et al. (2008), Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients. J. Immunol., 180(11):7221-9.
PMID: 18490721
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Binder M et al. (2007), Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab. Cancer Res., 67(8):3518-23.
PMID: 17440057
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Li QR et al. (2005), Interleukin-2alpha receptor in membrane lipid rafts. Transplant. Proc., 37(5):2395-7.
PMID: 15964425
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sandrin V et al. (2004), Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses. J. Virol., 78(13):7153-64.
PMID: 15194792

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Customer Reviews for Human CD25/IL-2R alpha Antibody (3)

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Human CD25/IL-2R alpha Antibody

Name: Katie Myers

Application: ELISA

Sample Tested: Serum

Species: Primate

Verified Customer | Posted 11/28/2022

Used as capture antibody in NHP ELISA
Human CD25/IL-2R alpha Antibody

Name: Anonymous

Application: ELISA

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 07/23/2019
Human CD25/IL-2 R alpha Antibody

Name: Chris Lyman

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 11/03/2017

This antibody served as both a capture and detection antibody in a sandwich ELISA developed to measure human serum and plasma samples.

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Human CD25/IL-2R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human CD25/IL-2R alpha Antibody

Scientific Data Examples for Human CD25/IL-2R alpha Antibody

Western Blot

Knockout Validated

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD25/IL-2R alpha

Citations for Human CD25/IL-2R alpha Antibody (4)

Customer Reviews for Human CD25/IL-2R alpha Antibody (3)

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Human CD25/IL-2R alpha Antibody

Human CD25/IL-2R alpha Antibody

Human CD25/IL-2 R alpha Antibody

Product Documents for Human CD25/IL-2R alpha Antibody

Product Datasheets

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