Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Human CCR2 Alexa Fluor® 700‑conjugated Antibody

Catalog # FAB151N | R&D Systems, Inc. a Bio-Techne Brand
Clone 48607 was used by HLDA to establish CD designation

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 48607

Product Summary for Human CCR2 Alexa Fluor® 700‑conjugated Antibody

Immunogen

NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868

Specificity

Detects human CCR2 transfectants but not the parental cell line or CCR5 transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCR2 Alexa Fluor® 700‑conjugated Antibody

Detection of CCR2 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCR2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB151N) or (B) Mouse IgG2BAlexa Fluor 700 Isotype Control (Catalog # IC0041N). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR2 in Human PBMC monocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CCR2 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB151N, filled histogram) or Mouse IgG2BAlexa Fluor 700 Isotype Control (IC0041N, open histogram). Staining was performed using Staining Membrane-associated Proteins protocol.

Applications for Human CCR2 Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood monocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using FAB151N in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

UniProt

Additional CCR2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCR2 Alexa Fluor® 700‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Setting the standard in quality research reagents