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Human Cathepsin D Antibody

Bio-Techne includes R&D Systems | Catalog # MAB10141

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MAB10141-100
MAB10141-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 984824

Product Summary for Human Cathepsin D Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin D
Leu21-Leu412
Accession # P07339

Specificity

Detects human Cathepsin D in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Cathepsin D Antibody

Cathepsin D antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

Cathepsin D in U937 Human Cell Line.

Cathepsin D was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human Cathepsin D Monoclonal Antibody (Catalog # MAB10141) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Cathepsin D Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample:

Immersion fixed U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB10141 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin D

Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (aa 1-18), a propeptide (aa 19-64), and a mature chain (aa 65‑412) (2‑4). The mature chain can be processed further to the light (aa 65‑161) and heavy (aa 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

References

  1. Conner et al. in Handbook of Proteolytic Enzymes Barrett (1998) Academic Press, San Diego, p. 828.
  2. Faust, et al. (1985) Proc. Natl. Acad. Sci. USA 82:4910.
  3. Westley and May (1987) Nucl. Acid Res. 15:3773.
  4. Redecker, et al. (1991) DNA Cell Biol. 10:423.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.

Alternate Names

CTSD

Entrez Gene IDs

1509 (Human); 13033 (Mouse)

Gene Symbol

CTSD

UniProt

Additional Cathepsin D Products

Product Documents for Human Cathepsin D Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cathepsin D Antibody

For research use only

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