Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

Detects human Cadherin-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cadherin-4, -6, -8, -12, -13,-17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Cadherin-11 Antibody

Detection of Cadherin‑11 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Cadherin-11 Monoclonal Antibody (Catalog # MAB17901, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Cells were stained in a buffer containing Ca²⁺and Mg²⁺.

Applications for Human Cadherin-11 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: PC‑3 human prostate cancer cell line stained in buffer containing Ca²⁺ and Mg²⁺

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).

References

Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
Kimura, Y. et al. (1995) Dev. Biol. 169:347.
McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Entrez Gene IDs

1009 (Human); 12552 (Mouse)

Gene Symbol

CDH11

UniProt

AAA35622

Additional Cadherin-11 Products

Product Documents for Human Cadherin-11 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cadherin-11 Antibody

For research use only

Citations
Reviews

Protocols
FAQs

Calculators
Flow Cytometry

Human Cadherin-11 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17901

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Cadherin-11 Antibodies »

Product Specifications

Immunogen

Specificity