Detects human Cadherin-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cadherin-4, -6, -8, -12, -13,-17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications

Application

Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).

References

Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
Kimura, Y. et al. (1995) Dev. Biol. 169:347.
McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Entrez Gene IDs

1009 (Human); 12552 (Mouse)

Gene Symbol

CDH11

UniProt

AAA35622

Additional Cadherin-11 Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Flow Cytometry

Human Cadherin-11 Alexa Fluor™ Plus 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB17901AFP594

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Cadherin-11 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications

CyTOF-ready

Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Shipping

Stability & Storage

Background: Cadherin-11

References

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Cadherin-11 Products

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices

CONTACT INFORMATION

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