Human BMP-2/BMP-4 Antibody

Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody.

Recombinant Human BMP-2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND₅₀ is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).

Detection of BMP-2/BMP-4 by Western Blot

Effects of neutralization of BMP2 and BMP4 on xenografted tumor growth induced by DragonCMT93 cells with or without Dragon over-expression were injected into C57BL/6 mice, followed by intraperitoneal injection of 2 μg/body weight of BMP2/4 antibody or IgG1 once every 2 days from the day of cell injection to 22 days after cell injection. A.Xenografted tumors were isolated from mice at 22 days after cell injection. B. The sizes of xenografted tumors were measured at days 7, 10, 13, 16 19 and 22 after cell injection (n = 6). P < 0.05, oeDra+anti-BMP2/4 vs oeDra+IgG. C. Representative images from Micro-PET/CT performed on mice at day 20 after cell injection: white circular outlines indicate xenografted tumors; arrows indicate SUV-MAX absorbance. D. Tumor volumes and SUV-MAX absorbance are quantified by Micro-PET/CT at day 20 after cell injection (n = 6). E. Phosphorylation levels of Erk1/2 and Smad1/5/8 in tumors collected at day 22 after cell injections (upper panel, Western blots; lower panel, densitometric analysis). F. Schematic diagram depicting Dragon action in regulating proliferation in colon cancer cells. Dragon/BMPs activate the Smad1/5/8 and Erk1/2 pathways via a mechanism yet to be determined and then promote colon cancer cell proliferation and tumor growth. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26029998), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human BMP-2/BMP-4 by Flow Cytometry

Transcriptional regulation of IRX3 and IRX5. (A) RQ-PCR analysis of HOXA10 in AML cell lines (left). Transcript levels are indicated in relation to OCI-AML3, and IRX3/IRX5-positive cell lines are indicated in red. RQ-PCR analysis of OCI-AML3 (above) and MEGAL (below) showed downregulation of IRX3 and IRX5 after siRNA-mediated knockdown of HOXA10 (right). (B) RQ-PCR analysis of BMP2 in AML cell lines (left). Transcript levels are indicated in relation to OCI-AML3. ELISA results for BMP2 protein levels in AML cell line supernatants are inserted. RQ-PCR analysis of OCI-AML3 treated with BMP2 resulted in the upregulation of IRX3 and IRX5 (right). (C) RQ-PCR analysis of OCI-AML3 after siRNA-mediated knockdown of JUNB (left) and SMAD4 (right) showed downregulation of IRX3 and IRX5. (D) RQ-PCR analysis of JUNB in AML cell lines (left). Transcript levels are indicated in relation to OCI-AML3. RQ-PCR analysis of OCI-AML3 treated with inhibitory BMP2-antibody resulted in downregulation of JUNB, IRX3, and IRX5, while HOXA10 was not significantly affected (right). p-values are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not significant). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35328612), licensed under a CC-BY license. Not internally tested by R&D Systems.