Human beta-Catenin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC13292A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 196624
Product Specifications
Immunogen
E. coli-derived recombinant human beta-Catenin
Ala2-Leu781
Accession # P35222
Ala2-Leu781
Accession # P35222
Specificity
Detects human, mouse, and rat beta-Catenin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human beta-Catenin APC-conjugated Antibody
Detection of beta‑Catenin in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta-Catenin APC-conjugated Monoclonal Antibody (Catalog # IC13292A, filled histogram) or isotype control antibody (Catalog # IC003A, open histo-gram). To facilitate intracellular staining, cells were fixed with 4% para-formaldehyde and permeabilized with Methanol. View our protocol for Staining Intracellular Molecules.Applications for Human beta-Catenin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with 4% paraformaldehyde and permeabilized with Methanol.
Sample: HeLa human cervical epithelial carcinoma cell line fixed with 4% paraformaldehyde and permeabilized with Methanol.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-Catenin
beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
UniProt
Additional beta-Catenin Products
Product Documents for Human beta-Catenin APC-conjugated Antibody
Product Specific Notices for Human beta-Catenin APC-conjugated Antibody
For research use only
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