S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3 (R&D Systems, Catalog # 3829-AN)
Ser17-Glu460
Accession # Q9Y5C1

Specificity

Detects human Angiopoietin-like 3 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-4, rhANGPTL4, recombinant mouse (rm) Angiopoietin-3, rmANGPTL3 and rmANGPTL4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

Human Angiopoietin-like Protein 3 / ANGPTL3 ELISA Standard Curve

Recombinant Human Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 3829-AN) was serially diluted and captured by Rat Anti-Human Angiopoietin-like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Angiopoietin-like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3829) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Angiopoietin-like 3 (Catalog # 3829-AN)

Human Angiopoietin-like Protein 3/ANGPTL3 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human Angiopoietin-like Protein 3/ANGPTL3 Antibody (Catalog # MAB38291)
Standard: Recombinant Human Angiopoietin-like 3 Protein, CF (Catalog # 3829-AN)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N‑terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C‑terminal fibrinogen-like domain that binds integrin alpha_v beta₃. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N‑terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C‑terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9Y5C1 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

For research use only

Citations
Reviews

Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3829

Key Product Details

Species Reactivity

Validated:

Cited:

Applications