Histone H2AX Antibody (CL5157) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44279
![Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]](https://resources.bio-techne.com/images/products/nbp3-44279_mouse-histone-h2ax-mab-cl5157-azide-and-bsa-free-282202513415628.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]")
![Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody [NBP3-44279]](https://resources.bio-techne.com/images/products/nbp3-44279_mouse-histone-h2ax-mab-cl5157-azide-and-bsa-free-282202513355863.jpg "Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody [NBP3-44279]")
![Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]](https://resources.bio-techne.com/images/products/nbp3-44279_mouse-histone-h2ax-mab-cl5157-azide-and-bsa-free-282202512182722.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL5157
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P16104, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Histone H2AX Antibody (CL5157) - Azide and BSA Free
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]
Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.![Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]](https://resources.bio-techne.com/images/products/nbp3-44279_mouse-histone-h2ax-mab-cl5157-azide-and-bsa-free-28220251235127.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]
Staining of human kidney shows strong nuclear positivity in renal tubules cells.![Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]](https://resources.bio-techne.com/images/products/nbp3-44279_mouse-histone-h2ax-mab-cl5157-azide-and-bsa-free-282202512405513.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]
Staining of human endometrium shows strong nuclear immunoreactivity in glandular and stromal cells.Applications for Histone H2AX Antibody (CL5157) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:20000 - 1:50000
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (CL5157) - Azide and BSA Free
Product Specific Notices for Histone H2AX Antibody (CL5157) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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