Histone H2AX Antibody (CL5157) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21157
![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.bio-techne.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315534342.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.bio-techne.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315531392.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
![Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -](https://resources.bio-techne.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315493273.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL5157
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P16104, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Histone H2AX Antibody (CL5157) - BSA Free
Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -
Staining of MCF7 cells using the Anti-H2AFX monoclonal antibody, showing specific staining in nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.bio-techne.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-4520231548527.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -
Staining of human endometrium shows strong nuclear immunoreactivity in glandular and stromal cells.![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.bio-techne.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315472814.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -
Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.Applications for Histone H2AX Antibody (CL5157) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:20000 - 1:50000
Immunohistochemistry-Paraffin
1:20000 - 1:50000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (CL5157) - BSA Free
Product Specific Notices for Histone H2AX Antibody (CL5157) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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