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GAD65/67 Antibody (L127/12) [FL594] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52409

Recombinant Monoclonal Antibody
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NBP3-52409

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

FL594

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # L127/12

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Fusion protein amino acids 1-594 (full-length) of human GAD65/67 (Uniprot#: Q99259) produced recombinantly in E. Coli

Specificity

Cross-reacts with GAD25, GAD44 and GAD65/GAD2

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

FL594 Excitation: 594 nm, Emission: 615nm.
Each order is a unique lot. For specialized requirements, please inquire for available options.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.

Applications for GAD65/67 Antibody (L127/12) [FL594] - Chimeric

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GAD65/67

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Alternate Names

67kD), EC 4.1.1, FLJ45882, glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 67 kDa isoform

Gene Symbol

GAD1

Additional GAD65/67 Products

Product Documents for GAD65/67 Antibody (L127/12) [FL594] - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAD65/67 Antibody (L127/12) [FL594] - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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