GAD65/67 Antibody (L127/12) - Chimeric - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52354

Recombinant Monoclonal Antibody
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NBP3-52354-100ul
NBP3-52354-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # L127/12

Format

BSA Free

Concentration

1 mg/ml

View all GAD65/67 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-594 (full-length) of human GAD65/67 (Uniprot#: Q99259) produced recombinantly in E. Coli

Specificity

Cross-reacts with GAD25, GAD44 and GAD65/GAD2

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images for GAD65/67 Antibody (L127/12) - Chimeric - BSA Free

Immunohistochemistry-Paraffin: GAD65/67 Antibody (L127/12) - Chimeric - BSA Free [NBP3-52354]

Immunohistochemistry-Paraffin: GAD65/67 Antibody (L127/12) - Chimeric - BSA Free [NBP3-52354]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with NBP3-52354 (green) at 1:100 dilution. DAPI (blue) shows cell nuclei. This antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 100X.
Western Blot: GAD65/67 Antibody (L127/12) - Chimeric - BSA Free [NBP3-52354]

Western Blot: GAD65/67 Antibody (L127/12) - Chimeric - BSA Free [NBP3-52354]

Western blotting of rat brain homogenate with GAD65/67 mouse monoclonal antibody (A) and NBP3-52354 (B). The mouse monoclonal antibody and chicken monoclonal antibody were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The chicken monoclonal antibody recognizes endogenous GAD65/67 with similar sensitivity as the mouse monoclonal antibodyin the rat brain homogenate at ~65-67 kDa.
Immunohistochemistry-Paraffin: GAD65/67 Antibody (L127/12) - Chimeric - BSA Free [NBP3-52354]

Immunohistochemistry-Paraffin: GAD65/67 Antibody (L127/12) - Chimeric - BSA Free [NBP3-52354]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with NBP3-52354 (green) at 1:100 dilution. DAPI (blue) shows cell nuclei. This antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 400X.

Applications for GAD65/67 Antibody (L127/12) - Chimeric - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD65/67

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Alternate Names

67kD), EC 4.1.1, FLJ45882, glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 67 kDa isoform

Gene Symbol

GAD1

Additional GAD65/67 Products

Product Documents for GAD65/67 Antibody (L127/12) - Chimeric - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GAD65/67 Antibody (L127/12) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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