Clusterin/APOJ Antibody (Hs-3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-552
![Western Blot: Clusterin/APOJ Antibody (Hs-3)BSA Free [NB500-552]](https://resources.bio-techne.com/images/products/Clusterin-APOJ-Antibody-Hs-3-Western-Blot-NB500-552-img0001.jpg "Western Blot: Clusterin/APOJ Antibody (Hs-3)BSA Free [NB500-552]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine (Negative), Canine (Negative), Feline (Negative), Porcine (Negative)
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # Hs-3
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity
Apolipoprotein J (Hs-3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Clusterin/APOJ Antibody (Hs-3) - BSA Free
Western Blot: Clusterin/APOJ Antibody (Hs-3)BSA Free [NB500-552]
Western Blot: Clusterin/APOJ Antibody (Hs-3) [NB500-552] - Analysis of clusterin in human seminal plasma using anti-clusterin (Hs-3) purified.![Clusterin/APOJ Antibody (Hs-3) - BSA Free Western Blot: Clusterin/APOJ Antibody (Hs-3) - BSA Free [NB500-552]](https://resources.bio-techne.com/images/products/nb500-552_mouse-monoclonal-clusterin-apoj-antibody-hs-3-purified-13120241629547.jpg "Western Blot: Clusterin/APOJ Antibody (Hs-3) - BSA Free [NB500-552]")
Western Blot: Clusterin/APOJ Antibody (Hs-3) - BSA Free [NB500-552]
Analysis of human clusterin using mouse monoclonal antibody Hs-3 on lysates of MCF-7 cell line and MOLT-4 cell line (clusterin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-clusterin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Clusterin was detected around 40 kDa.Applications for Clusterin/APOJ Antibody (Hs-3) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1 ug/ml
Application Notes
Use in Flow reported in scientific literature (PMID:33176829) Predicted molecular weight: 60 kDa.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Clusterin
Additional Clusterin Products
Product Documents for Clusterin/APOJ Antibody (Hs-3) - BSA Free
Product Specific Notices for Clusterin/APOJ Antibody (Hs-3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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