CD40/TNFRSF5 Antibody (A54 scFv) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81101CL1
Recombinant Monoclonal Antibody
![CD40/TNFRSF5 Antibody (A54 scFv) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nb100-1683cl1_rabbit-mdm2-hdm2-p-ser185-pab-corafluor-1-29920251458396.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Functional
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # A54 scFv
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A54 human anti-CD40/TNFRSF5 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcgamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Specificity
A54 binds specifically to the D1 domain of CD40/TNFRSF5 (and can only bind to the intact i.e. non-truncated version of CD40/TNFRSF5 and when an AD2 peptide tag has not been added) and can recognise CD40/TNFRSF5 on a B cell line (BJAB). This scFv binds to CD40/TNFRSF5 (immobilized by amine coupling) with a KD within the range of 40nM. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for CD40/TNFRSF5 Antibody (A54 scFv) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for CD40/TNFRSF5 Antibody (A54 scFv) [CoraFluor™ 1]
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-81101. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD40/TNFRSF5
Alternate Names
CD40, TNFRSF5
Gene Symbol
CD40
Additional CD40/TNFRSF5 Products
Product Documents for CD40/TNFRSF5 Antibody (A54 scFv) [CoraFluor™ 1]
Product Specific Notices for CD40/TNFRSF5 Antibody (A54 scFv) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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