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CD161 Antibody (B199.2) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-65298

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NB100-65298
NB100-65298-0.025mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B199.2

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for CD161 Antibody (B199.2) - BSA Free

Immunogen

NB100-65298 recognises the human CD161 cell surface antigen, expressed by almost all NK cells and with a small subset of CD3+ve T cells.

Localization

Cell Membrane

Specificity

CD161 (B199.2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD161 Antibody (B199.2) - BSA Free

Flow Cytometry: CD161 Antibody (B199.2) [NB100-65298] - Figure A. PE-A750 conjugated mouse anti human CD3 and Pacific Blue conjugated mouse IgG1 isotype control. Figure B. PE-A750 conjugated mouse anti human CD3 and Pacific Blue conjugated mouse anti human CD161. All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock.
Flow Cytometry: CD161 Antibody (B199.2) [NB100-65298] - Figure A. PE-A750 conjugated mouse anti human CD3 and A647 conjugated mouse IgG1 isotype control. Figure B. PE-750 conjugated mouse anti human CD3 and A647 conjugated mouse anti human CD161. All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock.

Applications for CD161 Antibody (B199.2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD161

Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.

Alternate Names

CD161, CLEC5B, KLRB1, Ly59, NKR, NKRP1, NKRP1A

Entrez Gene IDs

3820 (Human)

Gene Symbol

KLRB1

UniProt

Additional CD161 Products

Product Documents for CD161 Antibody (B199.2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD161 Antibody (B199.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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