Detects bovine TNF-alpha in ELISAs and Western blots. In direct ELISA, less than 20% cross-reactivity with recombinant human TNF-alpha, recombinant porcine TNF‑ alpha and recombinant rhesus macaque TNF-alpha, less than 10% cross reactivity with recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, recombinant feline TNF‑ alpha, recombinant equine TNF‑ alpha and recombinant cotton rat TNF-alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Bovine TNF-alpha Antibody

Bovine TNF-alpha ELISA Standard Curve

Recombinant Bovine TNF-alpha (2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Bovine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998).

Applications for Bovine TNF-alpha Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Bovine TNF-alpha (Catalog # 2279-BT)

Bovine TNF-alpha Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:

Detection Reagent: Bovine TNF-alpha Biotinylated Antibody (Catalog # BAF2279)
Standard: Recombinant Bovine TNF-alpha Protein (Catalog # 2279-BT)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF2279 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Bovine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD) (3). Within the ECD, bovine TNF-alpha shares 64%-83% sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

References

Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.
Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
Cludts, I. et al. (1993) Cytokine 5:336.
Tang, P. et al. (1996) Biochemistry 35:8216.
Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Black, R.A. et al. (1997) Nature 385:729.
Moss, M.L. et al. (1997) Nature 385:733.
Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
Romanatto, T. et al. (2007) Peptides 28:1050.
Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Gene Symbol

TNF

UniProt

Q06599

Additional TNF-alpha Products

Product Documents for Bovine TNF-alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Bovine TNF-alpha Antibody

For research use only

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