BARD1 Antibody (2A11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00000580-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2A11

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BARD1 Antibodies »

Product Specifications

Immunogen

BARD1 (NP_000456, 658 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGKVWK

Specificity

BARD1 - BRCA1 associated RING domain 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BARD1 Antibody (2A11) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: BARD1 Antibody (2A11) [H00000580-M01]

Immunocytochemistry/ Immunofluorescence: BARD1 Antibody (2A11) [H00000580-M01]

Immunocytochemistry/Immunofluorescence: BARD1 Antibody (2A11) [H00000580-M01] - Analysis of monoclonal antibody to BARD1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: BARD1 Antibody (2A11) [H00000580-M01] - Detection limit for recombinant GST tagged BARD1 is approximately 3ng/ml as a capture antibody.

Applications for BARD1 Antibody (2A11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Sandwich ELISA

1:100-1:2000
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BARD1

This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer.

Long Name

BRCA1 associated RING domain 1

Alternate Names

BARD-1, BRCA1 associated RING domain 1, BRCA1-associated RING domain gene 1, BRCA1-associated RING domain protein 1, EC 6.3.2.-

Entrez Gene IDs

580 (Human)

Gene Symbol

BARD1

UniProt

NP_000456 (Human)

Additional BARD1 Products

Product Documents for BARD1 Antibody (2A11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BARD1 Antibody (2A11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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