B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79932
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # ICOSL/3260
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human B7-H2/ICOSLG protein (around aa 23-149) (exact sequence is proprietary) (Uniprot: O75144)
Localization
Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79798).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free
Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932]
Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ICOSL-Monospecific Mouse Monoclonal Antibody (ICOSL/3260).Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932]
Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.Applications for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1 - 2 ug/mL
Immunohistochemistry-Paraffin
1 - 2 ug/mL
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: B7-H2
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
Additional B7-H2 Products
Product Documents for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free
Product Specific Notices for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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