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B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79932

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ICOSL/3260

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human B7-H2/ICOSLG protein (around aa 23-149) (exact sequence is proprietary) (Uniprot: O75144)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79798).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free

Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932]

Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932]

Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ICOSL-Monospecific Mouse Monoclonal Antibody (ICOSL/3260).
Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932]

Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932]

Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Applications for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1 - 2 ug/mL
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: B7-H2

B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Gene Symbol

ICOSLG

Additional B7-H2 Products

Product Documents for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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