B7-H2/ICOSLG Antibody (ICOSL/3260)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79798

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ICOSL/3260

Concentration

0.2 mg/ml

View all B7-H2 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human B7-H2/ICOSLG protein (around aa 23-149) (exact sequence is proprietary) (Uniprot: O75144)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79932)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for B7-H2/ICOSLG Antibody (ICOSL/3260)

Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) [NBP2-79798]

Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) [NBP2-79798]

Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) [NBP2-79798] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with B7-H2/ICOSLG Antibody (ICOSL/3260).
Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) [NBP2-79798]

Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) [NBP2-79798]

Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) [NBP2-79798] - Analysis of Protein Array containing more than 19,000 full-length human proteins using B7-H2/ICOSLG Antibody (ICOSL/3260). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for B7-H2/ICOSLG Antibody (ICOSL/3260)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: B7-H2

B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Gene Symbol

ICOSLG

UniProt

O75144

Additional B7-H2 Products

Product Documents for B7-H2/ICOSLG Antibody (ICOSL/3260)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B7-H2/ICOSLG Antibody (ICOSL/3260)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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