B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12027
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-16C10-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12027-img0001.jpg "Immunocytochemistry/ Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # 16C10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.
Specificity
This antibody binds human B7-1/CD80 and inhibits the B7.1:CD28 pathway.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Scientific Data Images for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027]
Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric [NBP3-12027] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 16C10 (NBP3-12027) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12027, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027] -
Flow Cytometry: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027] - Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12027 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: B7-1/CD80
Alternate Names
B71, CD80
Gene Symbol
CD80
Additional B7-1/CD80 Products
Product Documents for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free
Product Specific Notices for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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