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ACOT7 Antibody (1D5) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-22569

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D5

Format

BSA Free

Concentration

1 mg/ml

Product Summary for ACOT7 Antibody (1D5) - BSA Free

Immunogen

Recombinant human ACOT7 (1-370aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ACOT7 Antibody (1D5) - BSA Free

Western Blot: ACOT7 Antibody (1D5) [NBP2-22569] - The 293T cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/Immunofluorescence: ACOT7 Antibody (1D5) [NBP2-22569] - Analysis in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for ACOT7 Antibody (1D5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACOT7

ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.

Alternate Names

ACH1, acyl-CoA thioesterase 7CTE-IIa, BACHEC 3.1.2.2, brain acyl CoA hydrolase, Brain acyl-CoA hydrolase, CTE-IILACH, cytosolic acyl coenzyme A thioester hydrolase, hBACH, long chain, Long chain acyl-CoA thioester hydrolase

Gene Symbol

ACOT7

Additional ACOT7 Products

Product Documents for ACOT7 Antibody (1D5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACOT7 Antibody (1D5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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