ACBP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92457
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human ACBP (NP_001171513.1). MWGDLWLLPPASANPGTGTEAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ACBP Antibody - BSA Free
Western Blot: ACBP Antibody - BSA Free [NBP2-92457] -
Western Blot: ACBP Antibody - BSA Free [NBP2-92457] - Western blot analysis of various lysates, using ACBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Western Blot: ACBP Antibody - BSA Free [NBP2-92457] -
Western Blot: ACBP Antibody - BSA Free [NBP2-92457] - Western blot analysis of lysates from wild type (WT) and ACBP knockout (KO) 293T cells, using [KO Validated] ACBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunoprecipitation: ACBP Antibody - BSA Free [NBP2-92457] -
Immunoprecipitation: ACBP Antibody - BSA Free [NBP2-92457] - Immunoprecipitation of ACBP from 300 ug extracts of 293T cells was performed using 3 ug of ACBP Rabbit pAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using ACBP Rabbit pAb at a dilution of 1:1000.Applications for ACBP Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ACBP
Long Name
Acyl-CoA-binding protein
Alternate Names
DBI, Endozepine, EP
Gene Symbol
DBI
Additional ACBP Products
Product Documents for ACBP Antibody - BSA Free
Product Specific Notices for ACBP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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