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ABCG2/CD338 Antibody (3G8) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-22124

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G8

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for ABCG2/CD338 Antibody (3G8) - BSA Free

Immunogen

Purified recombinant fragment of human ABCG2 expressed in E. coli.

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60-70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCG2/CD338 Antibody (3G8) - BSA Free

Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.
Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.

Applications for ABCG2/CD338 Antibody (3G8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
In Western blot, a dimer can be seen at 60-70 kDa representing ABCG2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-22124 in the following applications:

Published Applications

Read 8 publications using NBP2-22124 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCG2

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Long Name

ATP-binding Cassette Transporter G2

Alternate Names

ABCP, BCRP, CD338, MXR

Entrez Gene IDs

9429 (Human)

Gene Symbol

ABCG2

UniProt

Additional ABCG2 Products

Product Documents for ABCG2/CD338 Antibody (3G8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABCG2/CD338 Antibody (3G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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