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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 03

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 14-3-3 beta Antibody (03) [Alexa Fluor® 350]

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta (Accession#: NP_003395.1; Met1-Asn246).

Reactivity Notes

Has cross-reactivity in ELISA with:

Cynomolgus YWHAB

No cross-reactivity in ELISA with:

E.coli cell lysate (BL21 cell line)

Specificity

Has cross-reactivity in ELISA with: Cynomolgus YWHABNo cross-reactivity in ELISA with: E.coli cell lysate (BL21 cell line)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for 14-3-3 beta Antibody (03) [Alexa Fluor® 350]

14-3-3 beta Antibody (03) [Alexa Fluor® 350]

14-3-3 beta Antibody (03) [Alexa Fluor® 350] [NBP3-06516AF350] -

14-3-3 beta Antibody (03) [Alexa Fluor® 350] [NBP3-06516AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for 14-3-3 beta Antibody (03) [Alexa Fluor® 350]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 14-3-3 beta

14-3-3 proteins constitute a highly conserved family of homo- and heterodimeric proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs containing phosphorylated serine or threonine residues. There are at least seven distinct 14-3-3 genes in vertebrates: alpha/beta, epsilon, eta, gamma, theta, sigma, and zeta/delta.

Alternate Names

1433 beta, GW128, HS1, YWHAB

Gene Symbol

YWHAB

Additional 14-3-3 beta Products

Product Documents for 14-3-3 beta Antibody (03) [Alexa Fluor® 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 beta Antibody (03) [Alexa Fluor® 350]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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