PARP Universal Colorimetric Assay Kit
R&D Systems, part of Bio-Techne | Catalog # 4677-096-K
For the in vitro screening of candidate PARP-1 inhibitors
Key Product Details
Features
Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
Key Benefits
- Colorimetric readout
- 96 strip-well format
- Sensitive – detects 10 mU PARP /well
- Assay Time ~3 hrs
Species
Multi-Species
Product Summary for PARP Universal Colorimetric Assay Kit
Why Use the PARP/Apoptosis Colorimetric Assay Kit?
This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.
Product Specifications
For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
Scientific Data Images for PARP Universal Colorimetric Assay Kit
Inhibition of PARP Activity by 3-aminobenzamide.
Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit). (B) The standard curve is used to translate absorbance values to activity units of PARP.Kit Contents for PARP Universal Colorimetric Assay Kit
- 3-Aminobenzamide
- PARP-HSA, 10 Units/µl
- 20X PARP Buffer
- 10X PARP Cocktail
- 10X Activated DNA
- 10X Strep-Diluent
- Histone-Coated Plate Natural Strip Well Plate
- Strep-HRP
- TACS-Sapphire
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PARP
PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Additional PARP Products
Product Documents for PARP Universal Colorimetric Assay Kit
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