HT PARP in vivo Pharmacodynamic ELISA Kit II

Name: HT PARP in vivo Pharmacodynamic ELISA Kit II
Brand: R&D Systems
SKU: 4520-096-K

R&D Systems, part of Bio-Techne | Catalog # 4520-096-K

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Citations (20)

Product Datasheet / COA

New Version Available - 4520B-096-K

For the quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumor lysates from different tissues, organs and xenografts.

This product has been discontinuation and replaced by catalog # 4520B-096-K

Key Product Details

Features:	Monitors in vivo PARP activity within cells based on levels of PAR.
Key Benefits:	Pre-coated 96 well capture antibody plates High signal to noise ratio Detection sensitivity of 2 pg/mL of PAR Broad linear dynamic range to 1,000 pg/mL Reduced inter-assay variability Validated assay that measures drug action on PARP in both in vivo and in vitro settings 96 test size

Discontinued Product

4520-096-K has been discontinued. An alternative/replacement product is available: 4520B-096-K. View all PARP products.

Technical Data
Citations / Reviews

Summary for HT PARP in vivo Pharmacodynamic ELISA Kit II

What is the HT PARP In Vivo Pharmacodynamic ELISA Kit II.

Immobilized PAR monoclonal antibody in the wells of a 96-well plate captures cellular PAR and PAR attached to proteins. Incubation with a polyclonal PAR detecting antibody, followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of cellular PAR.

Product Specifications

Quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumor lysates from different tissues, organs and xenografts.
Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
Facilitating development of PARP and PARG targeted therapeutics.

Kit Contents for HT PARP in vivo Pharmacodynamic ELISA Kit II

PAR Standard, 25 pg/µl
WIL2-NS Cell Lysate Control Standard, Low
WIL2-NS Cell Lysate Control Standard, Medium
WIL2-NS Cell Lysate Control Standard, High
PAR Polyclonal Detecting Antibody
Goat anti-Rabbit IgG-HRP
DNase I, 2 Units/µl
PARP Sample Buffer
PARP Cell Lysis Reagent
100X Magnesium Cation
PARP Antibody Diluent
20% SDS
Pre-coated white 96-stripwell plate and 5 sealers
PARP PeroxyGlow A
PARP PeroxyGlow B

Product Specifications for HT PARP in vivo Pharmacodynamic ELISA Kit II

Species

Multi-species

Scientific Data Examples for HT PARP in vivo Pharmacodynamic ELISA Kit II

	Measurement of PAR Levels in Jurkat Cells. The HT PARP In Vivo Pharmacodynamic Assay II (Catalog # 4520-096-K) was used to measure poly (ADP-ribose) (PAR) levels in cellular extracts from untreated Jurkat human acute T cell leukemia cells, Jurkat cells treated with PJ34, a potent PARP inhibitor, for 1.5 hours, and in Jurkat cell extracts incubated for 30 minutes with PAR glycohydrolase (PARG), an enzyme that digests PAR.

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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