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Multiplex immunoassays for biomarker research

Luminex High Performance Immunoassays

Pre-defined Luminex multiplex panels are designed using key analytes from chosen research areas. These precise and accurate Luminex panels are optimized with custom diluents and extensively validated by the experts that bring you the immunoassay industry gold standard QuantikineTM ELISA kits.

  • Correlated to the gold standard Quantikine ELISA
  • Validated for specificity, precision, stability, linearity, and recovery
  • Kits available for human and non-human primate analytes
  • Premix or user-mix
  • Consistent and accurate results from lot-to-lot
  • Extensive menu
  • Small sample volume (<50 uL)
  • Assays performed in under 5 hours

Luminex High Performance Assays Correlated to Gold Standard Quantikine ELISAs

Cell Culture Supernate From Adipocytes Figs

Cell culture supernate from adipocytes were analyzed using both Luminex High Performance Assays and Quantikine ELISAs Cell culture supernate from pre-adipocytes before and after differentiation were harvested at the time points indicated and frozen.  Following collection, samples were analyzed using Human Leptin and Human Total MMP-3 Quantikine ELISAs (blue bars; Catalog # DLP00 and DMP300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LBHS000 and LMPM000, respectively).

Cell Culture Supernate From Sw1353 Figs

Cell culture supernate from SW1353 human chondrosarcoma cells were analyzed using both Luminex High Performance Assays and Quantikine ELISAs.

Cell culture supernate from SW1353 cells were either unstimulated or stimulated with 5 ng/mL IL-1 alpha and 10 ng/mL OSM, harvested 24 hours later, and frozen.  Following collection, samples were analyzed using Human CXCL10/IP-10, Human Total MMP-3, and Human Pro-MMP-13 Quantikine ELISAs (blue bars; Catalog # DIP100DMP300, and DM1300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LBHS000 and LMPM000, respectively).

Superior Performance Over the Competition

Percentage Recovery of Luminex Panels in Serum

Human Luminex Performance Panel Recovery Competitor Comparison. Human XL Cytokine Luminex Performance Panel (Catalog #FCSTM18) run against similar cytokine panels from two leading competitors. Analytes spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices. Average percent recovery is shown for all common analytes. Red bars indicate design specifications for High-Performance Luminex assays (70%-130%).

Immunoassays for Classification of Th1, Th2, and Th17 CD4+ T Cell Differentiation

  • Differentiate CD4+ T cells into Th1, Th2, and Th17 cells
  • Assay cell culture supernates with R&D Systems Luminex panels
  • Measured cytokine profiles consistent with expected results
T cells are immune cells that can be used in cell therapies.
Luminex Assay Users Guide

Luminex Assays User Guide

Learn and understand how multiplexing with R&D Systems Luminex immunoassays can benefit your workflow. We have a clear and easy guide to Luminex multiplex immunoassays. 

  • What is a Luminex multiplex immunoassay
  • Benefits of multiplexing
  • How R&D Systems ensures performance and consistency
  • Running an assay
  • Data analysis
  • Best practices and techniques
Immunoassay Quality Fundamentals Guide

Immunoassay Quality Fundamentals

Get consistent reproducible data every time with R&D Systems Luminex High Performance Assays. Rigorous quality control testing of immunoassays to meet strict performance specifications including intra- and inter-assay precision, assay linearity, and spike recovery ensure data accuracy for all validated sample types.

  • Recovery
  • Linearity
  • Specificity
  • Consistency
Custom Luminex assay antibody, analyte and species options

Custom Luminex Services

Utilize R&D Systems astounding collection of in-house proteins and antibodies to design a Luminex multiplex immunoassay panel to fit your research needs.

  • New analyte development
  • Bead region conflict resolution
  • Bulk packaging
  • Lot sequestration