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Human CXCL10/IP-10 ELISA Kit - Quantikine Best Seller

Bio-Techne includes R&D Systems | Catalog # DIP100

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DIP100
PDIP100
SIP100

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 µL), Serum (75 µL), EDTA Plasma (75 µL), Heparin Plasma (75 µL), Saliva (10 µL), Urine (100 µL)

Sensitivity

4.46 pg/mL

Assay Range

7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Product Summary for Human CXCL10/IP-10 Quantikine ELISA Kit

The Quantikine Human IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IP-10 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IP-10.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human IP-10

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human IP-10 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)
Serum (n=60)89 38-361
EDTA plasma (n=35)9647-382
Heparin plasma (n=35)11052-450
Saliva (n=5)729292-1340

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Urine (n=18)17.267ND-49.7
ND=Non-detectable

Cell Culture Supernates:
Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 mM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human IP-10.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
Unstimulated29,77421,900
Stimulated 18,45611,091

THP-1 human acute monocyte leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were stimulated with 1.0 μg/mL recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells were incubated for an additional 18 hours. An aliquot of the cell culture supernate was removed, diluted 600-fold and assayed, and measured 164,640 pg/mL. 




Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 70.3 174 342 80.7 194 362
Standard Deviation 2.07 5.15 10.74 7.91 13.48 24.27
CV% 2.9 3.0 3.1 9.8 6.9 6.7

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 67.4 168 334 70.7 182 366
Standard Deviation 3.12 4.99 11.15 6.23 11.12 18.93
CV% 4.6 3.0 3.3 8.8 6.1 5.2

Recovery for Human CXCL10/IP-10 Quantikine ELISA Kit

The recovery of IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 88-101
EDTA Plasma (n=5) 99 90-109
Heparin Plasma (n=5) 99 87-113
Serum (n=5) 99 88-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IP-10 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL10/IP-10/CRG-2 ELISA Linearity

Scientific Data Images for Human CXCL10/IP-10 Quantikine ELISA Kit

Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD5K Standard Curve

Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD5K Standard Curve

Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD6Q Standard Curve

Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD6Q Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Alternate Names

CRG-2, CRG2, IP-10

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Gene Symbol

CXCL10

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Human CXCL10/IP-10 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL10/IP-10 Quantikine ELISA Kit

For research use only

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.