N OLLAS Epitope Tag Expression and Detection Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBK1-18296

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NBK1-18296
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Key Product Details

Species

Non-species specific

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Kit Type

Expression and Detection Kit

Product Specifications

Applications

Flow Cytometry (reported in scientific literature)

Scientific Data Images for N OLLAS Epitope Tag Expression and Detection Kit

Western Blot: N OLLAS Epitope Tag Expression and Detection Kit [NBK1-18296]

Western Blot: N OLLAS Epitope Tag Expression and Detection Kit [NBK1-18296]

Western Blot: N OLLAS Epitope Tag Kit [NBK1-18296] - Western Blot on OLLAS tagged SFO-p41 protein.

Kit Contents for N OLLAS Epitope Tag Expression and Detection Kit

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: N OLLAS Epitope Tag

The pCMV-N-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with an N-terminal OLLAS tag. Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

Alternate Names

OLLA-2

Additional N OLLAS Epitope Tag Products

Product Documents for N OLLAS Epitope Tag Expression and Detection Kit

Product Datasheets

Certificate of Analysis

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Product Specific Notices for N OLLAS Epitope Tag Expression and Detection Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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