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Key Product Details

Species Reactivity

Epitope Tag

Applications

Chromatin Immunoprecipitation Sequencing, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # L2

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for OLLAS Epitope Tag Antibody (L2) - BSA Free

Immunogen

OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for OLLAS Epitope Tag Antibody (L2) - BSA Free

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody.
Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - The A08a neuron receives arbor-specific synaptic inputs. Posterior view of a single A08a labeled by MultiColor FlpOut (MCFO), visualized by A08a-Gal4 > UAS-MCFO. Image using the DyLight 650 form of this antibody (NBP1-06713C).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.

Applications for OLLAS Epitope Tag Antibody (L2) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

reported in scientific literature (PMID 31175278)

Immunoblotting

reported in scientific literature (PMID 27600537)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10 - 1:100

Immunohistochemistry-Frozen

1:10 - 1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-06713 in the following applications:

Published Applications

Read 33 publications using NBP1-06713 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OLLAS Epitope Tag

OLLAS (Escherichia coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a relatively newly discovered tag which is a 14 amino acid sequence residing in the junction between E. coli OmpF protein (OFL/OmpF linker) and mLangerin extracellular domain. OLLAS Epitope Tag antibody (L2), also called MAb L2 or OLLA-2, is a novel rat IgG monoclonal antibody raised against newly identified OLLAS epitope (SGFANELGPRLMGK), with advantages in immunodetection applications. Additionally, this antibody is a rat IgG and can be used for immunostaining of mouse and human samples without direct labelling of fluorochrome or enzyme. Because anti-tag antibodies are mostly generated from mouse and rabbit, the OLLAS Epitope Tag antibody (L2) from rat IgG provides an extra advantage in immunodetection.

Alternate Names

E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2

Product Documents for OLLAS Epitope Tag Antibody (L2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OLLAS Epitope Tag Antibody (L2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for OLLAS Epitope Tag Antibody (L2) - BSA Free (NBP1-06713):

[[URL:https://www.novusbio.com/products/ollas-epitope-tag-antibody-l2_nbp1-06… Epitope Tag Antibody (L2)]]
Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 5 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the primary antibody (NBP1-06713) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Dilute the appropriate secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

FAQs for OLLAS Epitope Tag Antibody (L2) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I am interested in tagging proteins from E. coli and am wondering if ompF cross-reactivity is a problem?

    A: The OLLAS tag is not predicted to cross-react with any portion of ompF.

  • Q: Can you confirm if you measure the concentration of NBP1-06713 (anti-OLLAS epitope tag)?

    A: Unfortunately, we do not measure the concentration of our OLLAS tag antibody, as the product is provided unpurified.

Showing  1 - 2 of 2 FAQs Showing All
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