IPTG
Tocris Bioscience, part of Bio-Techne | Catalog # 5509
Used in cloning procedures with X-GAL
Key Product Details
Description
Used in cloning procedures with X-GAL
Product Description
IPTG is an used in combination with X-GAL (Cat. No. 5510) in cloning procedures requiring expression of β-galactosidase activity. Non-metabolizable analog of galactose.This product has <0.1% dioxane content.
Product Specifications
Molecular Weight
238.30
Formula
C9H18O5S
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
1-Methylethyl 1-thio-β-D-galactopyranoside
CAS Number
367-93-1
PubChem ID
656894
InChI Key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
SMILES
O[C@@H]1[C@H](O)[C@@H](O)[C@H](SC(C)C)O[C@@H]1CO
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 23.83 | 100 |
Preparing Stock Solutions
for IPTG
The following data is based on the product molecular weight 238.30
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.20 mL | 20.98 mL | 41.96 mL |
| 5 mM | 0.84 mL | 4.20 mL | 8.39 mL |
| 10 mM | 0.42 mL | 2.10 mL | 4.20 mL |
| 50 mM | 0.08 mL | 0.42 mL | 0.84 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for IPTG
For research use only
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