X-Gal
Tocris Bioscience, part of Bio-Techne | Catalog # 5510
Used in cloning procedures with IPTG
Discontinued Product
5510 has been discontinued.
View all Buffers, Chelators and Reagents products.
Key Product Details
Description
Used in cloning procedures with IPTG
Product Description
Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.Product Specifications
Molecular Weight
408.63
Formula
C14H15BrClNO6
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
CAS Number
7240-90-6
PubChem ID
65181
InChI Key
OPIFSICVWOWJMJ-AEOCFKNESA-N
SMILES
ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 40.86 | 100 |
Preparing Stock Solutions
for X-Gal
The following data is based on the product molecular weight 408.63
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.45 mL | 12.24 mL | 24.47 mL |
| 5 mM | 0.49 mL | 2.45 mL | 4.89 mL |
| 10 mM | 0.24 mL | 1.22 mL | 2.45 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.49 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for X-Gal
For research use only
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