5-Ethynyluridine
Tocris Bioscience, part of Bio-Techne | Catalog # 7206
Uridine analog, used for labeling RNA synthesis
Key Product Details
Description
Uridine analog, used for labeling RNA synthesis
Product Description
5-Ethynyluridine is a cell-permeable uridine analog. 5-Ethynyluridine can be used in vitro and in vivo to label newly synthesized RNA. 5-Ethynyluridine can be used to study changes in transcription.Product Specifications
Molecular Weight
268.23
Formula
C11H12N2O6
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
CAS Number
69075-42-9
PubChem ID
14885960
InChI Key
QCWBIPKYTBFWHH-FDDDBJFASA-N
SMILES
OC[C@H]1O[C@@H](N2C=C(C(NC2=O)=O)C#C)[C@@H]([C@@H]1O)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 13.41 | 50 | |
| DMSO | 26.82 | 100 |
Preparing Stock Solutions
for 5-Ethynyluridine
The following data is based on the product molecular weight 268.23
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.73 mL | 18.64 mL | 37.28 mL |
| 5 mM | 0.75 mL | 3.73 mL | 7.46 mL |
| 10 mM | 0.37 mL | 1.86 mL | 3.73 mL |
| 50 mM | 0.07 mL | 0.37 mL | 0.75 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Van't Sant In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol.Commun. 2021 PMID: 34020718
- Nainar An optimized chemical-genetic method for cell-specific metabolic labeling of RNA. Nat.Methods 2020 PMID: 32015544
Product Specific Notices for 5-Ethynyluridine
For research use only
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