Mouse Mesenchymal Stem Cell Marker Antibody Panel
Catalog # SC018 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, mouse mesenchymal stem cell marker expression can be assessed using the following procedure:
- Incubate cells with the provided antibodies for 30 minutes
- Wash the cells
- Incubate the cells with fluorochrome-conjugated secondary antibodies
- Analyze samples by flow cytometry
Reagents Supplied in the Mouse Mesenchymal Stem Cell Marker Antibody Panel (Catalog # SC018):
Positive MSC Markers
- Rat Anti-Mouse Sca-1 IgG2A Monoclonal Antibody
- Rat Anti-Mouse CD29 IgG2A Monoclonal Antibody
- Sheep Anti-Mouse/Rat CD44 Polyclonal Antibody
- Rat Anti-Mouse CD73 IgG2A Monoclonal Antibody
- Rat Anti-Mouse CD105 IgG2A Monoclonal Antibody
- Rat Anti-Mouse CD106 IgG2A Monoclonal Antibody
Negative MSC Markers
- Rat Anti-Mouse CD11b IgG2B Monoclonal Antibody
- Rat Anti-Mouse CD45 IgG2B Monoclonal Antibody
Note: These antibodies have been tested for immunocytochemistry using human induced pluripotent stem cells grown either on irradiated mouse embryonic fibroblast feeder cells (Catalog # PSC001) or in feeder-free conditions. Each antibody is supplied as a 50X stock; enough for 25 assays when used in 500 µL staining volume per assay.
Reagents
- Flow Cytometry Staining Buffer (Catalog # FC001)
- Rat IgG2A Isotype control (Catalog # MAB006)
- Rat IgG2B Isotype control (Catalog # MAB0061)
- Secondary developing reagents (Catalog # F0113, F0104B, F0105B, and F0115)
- Sterile PBS
Materials
- Flow Cytometry Tubes
- Serological pipettes
Equipment
- Benchtop centrifuge
Staining of Mouse MSC Surface Markers
Perform a cell count on harvested cells.
Resuspend harvested cells in Flow Cytometry Staining Buffer.

Aliquot 90 μL of the cells into 5 mL flow cytometry tubes.

Add 10 μL of antibody or isotype control (or a previously titrated amount).
Vortex and incubate samples for 30 minutes at room temperature.

Centrifuge samples at 300 x g for 5 minutes.
Wash the samples three times with Flow Cytometry Staining Buffer.
Resuspend each sample in 100 μL of Flow Cytometry Staining Buffer.

Add 10 μL of a fluorochrome-conjugated secondary antibody (or a previously titrated amount).
Incubate for 30 minutes at room temperature in the dark.

Centrifuge the samples at 300 x g for 5 minutes.
Wash the sample two times in 2 mL of Flow Cytometry Staining Buffer.
Resuspend the cells in 200-400 μL of Flow Cytometry Staining Buffer.

Analyze the cells by flow cytometry.

Citations for Mouse Mesenchymal Stem Cell Marker Antibody Panel (17)
Citations are publications that use Bio-Techne products. Selected citations for Mouse Mesenchymal Stem Cell Marker Antibody Panel include:
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Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryM Tfilin et al. (2023-02-24), Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice International Journal of Molecular Sciences, 2023-024(5).
PMID: 36901920 -
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow CytometryS Kang et al. (2023-02-01), Adipose-derived mesenchymal stem cells promote salivary duct regeneration via a paracrine effect Journal of oral biosciences, 2023-00(0).
PMID: 36736698 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryN Gobshtis et al. (2019-12-11), Transplantation of mesenchymal stem cells causes long-term alleviation of schizophrenia-like behaviour coupled with increased neurogenesis Mol. Psychiatry, 2019-10(0).
PMID: 31827249 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryM Harmati et al. (2019-10-25), Small extracellular vesicles convey the stress-induced adaptive responses of melanoma cells Sci Rep, 2019-19(1):15329.
PMID: 31653931 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryR Fajka-Boja et al. (2018-09-05), Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells BMC Cancer, 2018-018(1):872.
PMID: 30185144 -
Species: Mouse
Sample Types: Whole Cells
J Xu et al. (2018-02-09), Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells Stem Cells, 2018-00(0).
PMID: 29377497 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryA Filipa Gon et al. (2017-11-13), Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells Nat Commun, 2017-18(1):1466.
PMID: 29133867 -
Species: Mouse
Sample Types: Whole Cells
N Gobshtis et al. (2017-03-14), Transplantation of mesenchymal stem cells reverses behavioural deficits and impaired neurogenesis caused by prenatal exposure to valproic acid Oncotarget, 2017-08(11):17443-17452.
PMID: 28407680 -
Species: Mouse
Sample Types: Whole Cells
YY Mot et al. (2017-01-23), Synergistic antibacterial effect of co-administering adipose-derived mesenchymal stromal cells and Ophiophagus hannah L-amino acid oxidase in a mouse model of methicillin-resistant Staphylococcus aureus-infected wounds Stem Cell Res Ther, 2017-08(1):5.
PMID: 28114965 -
Species: Mouse
Sample Types: Whole Cells
Muruganandan S et al. (2016-11-11), Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation. Stem Cells, 2016-135(3):711-724.
PMID: 27733019 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCYoshimatsu G et al. (2015-02-13), The co-transplantation of bone marrow derived mesenchymal stem cells reduced inflammation in intramuscular islet transplantation. PLoS ONE, 2015-010(2):e0117561.
PMID: 25679812 -
Species: Mouse
Sample Types: Whole Cells
Wang Z et al. (2015-02-01), Engineered mesenchymal stem cells with enhanced tropism and paracrine secretion of cytokines and growth factors to treat traumatic brain injury. Stem Cells, 2015-033(2):456-67.
PMID: 25346537 -
Species: Mouse
Sample Types: Whole Cells
Wu H et al. (2014-03-19), Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis. PLoS ONE, 2014-09(3):e90883.
PMID: 24646687 -
Species: Mouse
Sample Types: Whole Cells
Donnelly J et al. (2013-11-08), Gastritis promotes an activated bone marrow-derived mesenchymal stem cell with a phenotype reminiscent of a cancer-promoting cell. Dig Dis Sci, 2013-159(3):569-82.
PMID: 24202649 -
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHCHorton J et al. (2013-10-01), Mesenchymal stem cells inhibit cutaneous radiation-induced fibrosis by suppressing chronic inflammation. Stem Cells, 2013-131(10):2231-41.
PMID: 23897677 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryDonnelly J et al. (2013-09-19), Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS ONE, 2013-08(9):e75225.
PMID: 24069395 -
Species: Mouse
Sample Types: Whole Cells
Chartoff EH et al. (2011-11-15), Detection of intranasally delivered bone marrow-derived mesenchymal stromal cells in the lesioned mouse brain: a cautionary report. Stem Cells Int, 2011-12011(0):586586.
PMID: 22190964
There are no citations that match your criteria.