Human Mesenchymal Stem Cell Marker Antibody Panel
Catalog # SC017 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, human mesenchymal stem cell marker expression can be assessed using the following procedure:
- Incubate cells with the provided antibodies for 30 minutes
- Wash the cells
- Incubate the cells with fluorochrome-conjugated secondary antibodies
- Analyze samples by flow cytometry
Reagents Supplied in the Human Multipotent Mesenchymal Stromal Cell Marker Antibody Panel (Catalog # SC017):
Positive MSC Markers
- Mouse Anti-Human Stro-1 IgM gamma Monoclonal Antibody
- Mouse Anti-Human CD44 IgG2A Monoclonal Antibody
- Mouse Anti-Human CD-90 IgG2A Monoclonal Antibody
- Mouse Anti-Human CD105 IgG1 Monoclonal Antibody
- Mouse Anti-Human CD106 IgG1 Monoclonal Antibody
- Mouse Anti-Human CD146 IgG1 Monoclonal Antibody
- Mouse Anti-Human CD166 IgG1 Monoclonal Antibody
Negative MSC Markers
- Mouse Anti-Human CD19 IGg1 Monoclonal Antibody
- Mouse Anti-Human CD45 IGg1 Monoclonal Antibody
Reagents
- Flow Cytometry Staining Buffer (Catalog # FC001)
- Mouse IgG1 Isotype Control (Catalog # MAB002)
- Mouse IgG2A Isotype Control (Catalog # MAB003)
- Secondary developing reagents (Catalog # F0101B, F0102B, F0103B, F0114, F0116, F0117, F0118, and F0119)
- Sterile PBS
Materials
- Flow Cytometry Tubes
- Serological pipettes
Equipment
- Benchtop centrifuge
Staining of Mesenchymal Stem Cell Surface Markers
Perform a cell count on harvested cells.
Resuspend cells in Flow Cytometry Staining Buffer.

Aliquot 90 μl of the cells into 5 mL flow cytometry tubes.

Add 10 μL of antibody or isotype control (or a previously titrated amount).
Vortex and incubate for 30 minutes at room temperature.

Centrifuge samples at 300 x g for 5 minutes.
Wash the samples three times with Flow Cytometry Staining Buffer.
Resuspend each sample in 200 μL of Flow Cytometry Staining Buffer.

Add 10 μL of a fluorochrome-conjugated secondary antibody (or a previously titrated amount).
Incubate for 30 minutes at room temperature in the dark.

Centrifuge the samples at 300 x g for 5 minutes.
Wash the samples with Flow Cytometry Staining Buffer.
Resuspend the cells in 200-400 μL of Flow Cytometry Staining Buffer.

Analyze the cells by flow cytometry.

Citations for Human Mesenchymal Stem Cell Marker Antibody Panel (3)
Citations are publications that use Bio-Techne products. Selected citations for Human Mesenchymal Stem Cell Marker Antibody Panel include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryR Polak et al. (2018-10-31), Autophagy inhibition as a potential future targeted therapy for ETV6-RUNX1 driven B-cell precursor acute lymphoblastic leukemia Haematologica, 2018-10(0).
PMID: 30381299 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHopper N et al. (2015-08-07), Peripheral Blood Mononuclear Cells Enhance Cartilage Repair in in vivo Osteochondral Defect Model. PLoS ONE, 2015-010(8):e0133937.
PMID: 26252391 -
Species: Human
Sample Types: Whole Cells
Maria O et al. (2011-02-08), Matrigel improves functional properties of primary human salivary gland cells. Tissue Eng Part A, 2011-017(9):1229-38.
PMID: 21189069
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Customer Reviews for Human Mesenchymal Stem Cell Marker Antibody Panel (2)
Customer Images
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Verified Customer | Posted 02/13/2020
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Verified Customer | Posted 09/10/2019Need optimization of your specific cell types.
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