WS 6

Name: WS 6
Brand: Tocris Bioscience
SKU: 7791

Tocris Bioscience, part of Bio-Techne | Catalog # 7791

COA / SDS

Induces growth and proliferation of pancreatic islet cells, murine totipotent stem cells, GABAergic neurons and hippocampal neurons

Key Product Details

Description:	Induces growth and proliferation of pancreatic islet cells, murine totipotent stem cells, GABAergic neurons and hippocampal neurons
Chemical Name:	4-[[6-[(Cyclopropylcarbonyl)amino]-4-pyrimidinyl]oxy]-N-[4-[(4-methyl-1-piperazinyl)methyl]-3-(trifluoromethyl)phenyl]benzeneacetamide
Purity:	≥98% (HPLC)
Molecular Weight:	568.59

View Full Technical Details for WS 6

Technical Data
Citations

Biological Activity for WS 6

WS 6 induces growth and proliferation of cultured islet α and β cells in vitro (EC₅₀ = 0.28 μM at β cells). Inhibits growth of FLT3-ITD⁺ AML and K562 CML cells (IC₅₀ values are <0.24 nM and 40.48 nM, respectively). Reduces blood glucose in an animal model of type 1 diabetes. In combination with TTNPB (Cat. No. 0761) and 1-azakenpaullone, WS 6 induces and maintains murine totipotent stem cells. In an animal model of depression WS 6 induces dendrite complexity, enhances hippocampal neurogenesis, increases GABAergic neurons and GABA expression, and displays antidepressant and anxiolytic activity. WS 6 is predicted to be a receptor tyrosine kinase inhibitor.

Technical Data for WS 6

M. Wt	568.59
Formula	C₂₉H₃₁F₃N₆O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1421227-53-3
PubChem ID	71566751
InChi Key	FTODTDQFHDJWIQ-UHFFFAOYSA-N
Smiles	O=C(NC1=CC=C(C(=C1)C(F)(F)F)CN2CCN(C)CC2)CC3=CC=C(OC4=NC=NC(=C4)NC(=O)C5CC5)C=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for WS 6

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	56.86	100
	ethanol	56.86	100

Preparing Stock Solutions for WS 6

The following data is based on the product molecular weight 568.59.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.76 mL	8.79 mL	17.59 mL
5 mM	0.35 mL	1.76 mL	3.52 mL
10 mM	0.18 mL	0.88 mL	1.76 mL
50 mM	0.04 mL	0.18 mL	0.35 mL

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References for WS 6

References are publications that support the biological activity of the product.

Bregante Efficacy and synergy of small molecule inhibitors targeting FLT3-ITD+ acute myeloid leukemia. Cancers (Basel) 2021 PMID: 34944800
Shen Small-molecule inducer of β cell proliferation identified by high-throughput screening. J.Am.Chem.Soc. 2013 PMID: 23330637
Zhang WS6 induces adult hippocampal neurogenesis in correlation to its antidepressant effect on the alleviation of depressive-like behaviors of rats. Neuroscience 2021 PMID: 34455011
Menhaji-Klotz and Price Small molecules that promote regenerative repair for pancreatic and cardiovascular health. Bioorg.Med.Chem.Lett. 2015 PMID: 26508547
Hu Induction of mouse totipotent stem cells by a defined chemical cocktail. Nature 2022 PMID: 35728625