Triptolide

Name: Triptolide
Brand: Tocris Bioscience
SKU: 3253
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3253

Citations (7)

(1)

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Key Product Details

Description:	Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive
Chemical Name:	(3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one
Purity:	≥98% (HPLC)
Molecular Weight:	360.4

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Biological Activity for Triptolide

Triptolide inhibits DNA-dependent ATPase activity of XBP and induces inhibition of RNA polymerase II (RNAPII)-mediated transcription (IC₅₀ = 200 nM). Selective for RNAPII over RNAPI and RNAPIII. Triptolide blocks RNA synthesis in HeLa cells (IC₅₀ = 62 nM); exhibits potent antiproliferative activity in 60 cancer cell lines (average IC₅₀ = 12 nM) and induces apoptosis by blocking TNF-α-mediated c-IAP1 and c-IAP2 induction. Also displays immunosuppressive and anti-inflammatory activity.

Compound Libraries for Triptolide

Triptolide is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Triptolide

M. Wt	360.4
Formula	C₂₀H₂₄O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	38748-32-2
PubChem ID	107985
InChi Key	DFBIRQPKNDILPW-CIVMWXNOSA-N
Smiles	O=C1C2=C([C@@](C[C@H]5[C@]4(O5)[C@](O6)3[C@@H]6[C@H]7[C@](C(C)C)(O7)[C@H]4O)([H])[C@]3(C)CC2)CO1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Triptolide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.21	20

Preparing Stock Solutions for Triptolide

The following data is based on the product molecular weight 360.4.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	13.87 mL	69.37 mL	138.73 mL
1 mM	2.77 mL	13.87 mL	27.75 mL
2 mM	1.39 mL	6.94 mL	13.87 mL
10 mM	0.28 mL	1.39 mL	2.77 mL

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References for Triptolide

References are publications that support the biological activity of the product.

Titov XPB, a subunit of TFIIH, is a target of the natural product triptolide. Nat.Chem.Biol. 2011 PMID: 21278739
Qui Immunosuppressant PG490 (Triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-κB transcriptional activation. J.Biol.Chem. 1999 PMID: 10224109
Lee PG490 (Triptolide) cooperates with tumor necrosis factor-α to induce apoptosis in tumor cells. J.Biol.Chem. 1999 PMID: 10224110

Citations for Triptolide (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Triptolide include:

Shao et al. (2019) Reporter-ChIP-nexus reveals strong contribution of the Drosophila initiator sequence to RNA polymerase pausing. Elife. 8
PMID: 31021316
Tettey et al. (2019) A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing. Cell Rep. 27:3770
PMID: 31242411
Bowry et al. (2018) BET Inhibition Induces HEXIM1- and RAD51-Dependent Conflicts between Transcription and Replication. Cell Rep. 25:2061
PMID: 30463005
Liao and Mizzen (2017) Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation. Epigenetics Chromatin. 10:29
PMID: 28539972
Zhao et al. (2015) Hyaluronic acid prevents immunosuppressive drug-induced ovarian damage via up-regulating PGRMC1 expression. Sci Rep. 5:7647
PMID: 25558795
Filone et al. (2014) The master regulator of the cellular stress response (HSF1) is critical for orthopoxvirus infection. PLoS Pathog. 10:e1003904
PMID: 24516381
Vollmer et al. (2012) Anti-inflammatory effects of mapracorat, a novel selective glucocorticoid receptor agonist, is partially mediated by MAP kinase phosphatase-1 (MKP-1). J Biol Chem. 287:35212
PMID: 22898817

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Customer Reviews for Triptolide (1)

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Name: Anonymous

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: U2OS

triptolide (1 μM)

I used short treatments with the transcription inhibitors triptolide. These inhibited ongoing RNA synthesis and increased replication fork speeds specifically in the presence of JQ1

PMID: 30463005

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Product Documents for Triptolide

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.