Trichostatin A

Product Description

Trichostatin A is a selective and potent inhibitor of class I and II histone deacetylases (HDAC) (K_i = 3.4 nM, IC₅₀ values are 4.99 nM, 5.21 nM, 16.4 nM, 24.3 nM, 27.6 nM for HDAC1, 3, 6, 10 and 4, respectively). Trichostatin A induces accelerated dedifferentiation of primordial germ cells (PGCs) into embryonic germ (EG) cells. Trichostatin A potently inhibits a range of breast cancer and melanoma cell lines (IC₅₀ = 29-400 nM) and induces apoptosis in prostate cancer cells. In an animal model of Huntington's disease, inhibition of HDAC by Trichostatin A rescues memory deficits and increases expression of CREB/CBP target genes in mutant mice. Activates autophagy, increases vesicular transport of BDNF (Cat. No. 2837) and is active in vivo.Trichostatin A enhances CRISPR-Cas9 gene editing of human induced pluripotent stem cells (iPSCs).

Solubility

Preparing Stock Solutions for Trichostatin A

The following data is based on the product molecular weight 302.37

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.17 mM	19.45 mL	97.27 mL	194.54 mL
0.85 mM	3.89 mL	19.45 mL	38.91 mL
1.7 mM	1.95 mL	9.73 mL	19.45 mL
8.5 mM	0.39 mL	1.95 mL	3.89 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Molugu Trichostatin A for efficient CRISPR-Cas9 gene editing of human pluripotent stem cells. CRISPR J. 2023 PMID: 37676985
• Tatamiya Isozyme-selective activity of the HDAC inhibitor MS-275. Proc.Amer.Assoc.Cancer Res.
• Giralt Long-term memory deficits in Huntington's disease are associated with reduced CBP histone acetylase activity. Hum.Mol.Genet. 2012 PMID: 22116937
• Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID:  28529316
• Wharton Inhibition of mitogenesis in Balb/c-3T3 cells by trichostatin A. J.Biol.Chem. 2000 PMID: 10945992
• Vigushin Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin.Cancer Res. 2001 PMID: 11309348
• Yoshida Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. J.Biol.Chem. 1990 PMID: 2211619
• Durcova-Hills Reprogramming primordial germ cells into pluripotent stem cells. PloS ONE 2008 PMID: 18953407